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[Objective]The paper was to develop a rapid method for the detection of spring Viremia of carp virus(SVCV).[Method]The specific primers were designed by targeting the G gene of SVCV.The recombinase polymerase amplification(RPA)assay for detecting SVCV was estab-lished by optimizing the reaction conditions.The optimal amplification temperature of RPA assay was 30℃,and the test could be finished within 20 min.[Result]The method was specific with no cross-reaction with other common fish infectious viruses.Sensitivity test showed that the lowest detection limit of the method was 89.2 copies/μL,higher than that of traditional RT-PCR.Moreover,a total of 80 clinical samples were detected by RPA and RT-PCR,respectively.The weak positive samples tested by RT-PCR could be detectable with RPA,indicating that RPA assay could be used in clinical detection.[Conclusion]The method established is rapid,simple,specific and sensitive for testing SVCV,and it will be widely used in grassroots laboratory and on-site inspection.
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篇名 Establishment of Recombinase Polymerase Amplification for Rapid Detection of Spring Viremia of Carp Virus(SVCV)
来源期刊 动物与饲料科学:英文版 学科 农学
关键词 Recombinase polymerase amplification Rapid detection Spring viremia of carp virus(SVCV)
年,卷(期) 2020,(2) 所属期刊栏目
研究方向 页码范围 29-32
页数 4页 分类号 S94
字数 语种
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节点文献
Recombinase
polymerase
amplification
Rapid
detection
Spring
viremia
of
carp
virus(SVCV)
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
动物与饲料科学:英文版
双月刊
1943-9911
安徽省合肥市农科南路40号
出版文献量(篇)
46
总下载数(次)
0
总被引数(次)
0
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