论文降重
免费查重- 钛学术文献服务平台 \
- 学术期刊 \
- 综合期刊 \
- 其它期刊 \
- 动物与饲料科学：英文版期刊 \
Preliminary Screening and Characterization of Microsatellite Markers in RAD-seq Data of Datnioides pulcher
Preliminary Screening and Characterization of Microsatellite Markers in RAD-seq Data of Datnioides pulcher
基本信息来源于合作网站，原文需代理用户跳转至来源网站获取
摘要：
[Objective]The paper was to develop the microsatellite markers of Datnioides pulcher,and to screen polymorphic SSR primers.[Method]D.pulcher was performed reduced-representation genome sequencing using RAD-seq(Restriction-site associated DNA sequencing).[Result]A total of 13308806 high-quality clean reads(HQ clean reads)lengths were obtained and 26359 simple sequence repeats(SSR)loci were detected in the obtained sequence by SSR search software,which consisted of 496 repeat motifs.The most common types of repeat were A/T in single base repeat sequence,and AC/GT and A G/GT repeat units in di-base repeat sequence.AGG/CCT,AGC/CTG and AGG/CCT were the dominant types in tri-base repeat sequence.In quantity,the di-base repeat type SSR loci were the largest(19492),accounting for 73.95%of the total SSR loci.Except for mono-nucleotide type,the repetition frequency of SSR loci was mainly ranged from 4 to 9,and the number of polymorphic SSR loci was 2783.About 20066 pairs of SSR primers were successfully designed with Primer 5.0.The success rate of primer design was 76.13%.According to the repetition type and repetition number in the sequencing data,100 di-to penta-base repetition type SSRs were randomly selected and validated by PCR and polyacrylamide gel in six D.pulcher samples.A total of 73 pairs of primers were amplified by electrophoresis,and 20 of them were polymorphic.[Conclusion]The developed SSR sequences can be used for genetic analysis,genetic map construction and molecular marker assisted breeding of D.pulcher and its related species.
推荐文章
RAD-seq技术及其在昆虫学研究中的应用
RAD-seq
昆虫学
种群遗传学
物种界定
系统发育
遗传连锁图谱
一种用于桑RAD－Seq高通量测序的基因组DNA提取方法
桑树
RAD-Seq
基因组DNA提取
RAD-seq技术在大白猪繁殖性状基因组选择上的应用
简化基因组测序技术(RAD-seq)
基因组选择
繁殖性状
最佳线性无偏预测(BLUP)
基于RAD-seq技术的鹅掌楸基因组SNP标记开发
北美鹅掌楸
鹅掌楸
限制性位点关联DNA测序(RAD-seq)技术
SNP标记
遗传连锁图谱
内容分析
关键词云
关键词热度
相关文献总数
(/次)
(/年)
文献信息
| 篇名 | Preliminary Screening and Characterization of Microsatellite Markers in RAD-seq Data of Datnioides pulcher | ||
| 来源期刊 | 动物与饲料科学:英文版 | 学科 | 农学 |
| 关键词 | Datnioides pulcher RAD-seq Simple sequence repeats | ||
| 年，卷（期） | 2020,（2） | 所属期刊栏目 | |
| 研究方向 | 页码范围 | 23-28 | |
| 页数 | 6页 | 分类号 | S51 |
| 字数 | 语种 | ||
| DOI | |||
五维指标
引文网络
引文网络
二级参考文献 (0)
共引文献 (0)
参考文献 (0)
节点文献
引证文献 (0)
同被引文献 (0)
二级引证文献 (0)
2020(0)
- 参考文献(0)
- 二级参考文献(0)
- 引证文献(0)
- 二级引证文献(0)
研究主题发展历程
节点文献
Datnioides
pulcher
RAD-seq
Simple
sequence
repeats
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
动物与饲料科学：英文版
主办单位：
吴楚(美中)科技文化传媒公司
出版周期：
双月刊
ISSN：
1943-9911
CN：
开本：
出版地：
安徽省合肥市农科南路40号
邮发代号：
创刊时间：
语种：
出版文献量（篇）
46
总下载数（次）
0
总被引数（次）
0
期刊文献
相关文献
推荐文献
- 期刊分类
- 期刊（年）
- 期刊（期）
- 期刊推荐
