Effects of Different Extraction and Purification Methods on the Acquisition of Phycoerythrin from Porphyridium purpureum

Jinglong LI; Shengxin NIE; Yuanchao XU; Zhen LIU

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Effects of Different Extraction and Purification Methods on the Acquisition of Phycoerythrin from Porphyridium purpureum

Effects of Different Extraction and Purification Methods on the Acquisition of Phycoerythrin from Porphyridium purpureum

作者：

Jinglong LI Shengxin NIE Yuanchao XU Zhen LIU

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PHYCOERYTHRIN

EXTRACTION

PURIFICATION

IDENTIFICATION

摘要：

Phycoerythrin, as the main light-harvesting antenna in Porphyridium purpureum , exists at the outermost end of the phycobilisome. It has advantages of good fluorescence intensity, anti-oxidation, scavenging free radicals, and high chroma, so it has been widely used in food, cosmetics, pharmaceuticals, and other industries. In this study, the effects of different extraction (ultrasonic breaking method, bead grinding method, liquid nitrogen grinding method, and freezing-thawing method) and purification methods (salting out method, ultrafiltration method, and combination of salting out and ultrafiltration method) on the acquisition of phycoerythrin from P. purpureum were studied, and the characteristics of phycoerythrin in the P. purpureum were identified. The results showed that the freezing-thawing method could extract phycoerythrin from the powder of P. purpureum to the utmost extent, and the concentration of the extracted phycoerythrin was up to 0.036 g/L. The salting out method could most effectively purify phycoerythrin, and the purity index was 2.216. The identification of phycoerythrin by ultraviolet absorption spectroscopy and fluorescence spectroscopy indicated that the phycoerythrin had the maximum absorption peak at 545 nm, and the maximum Stokes shift was up to 79 nm. Due to its high fluorescence characteristics, it can be used as a fluorescent marker in the fields of molecular biology and clinical medicine, and can also be used as a good photosensitizer in tumor therapy.

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篇名	Effects of Different Extraction and Purification Methods on the Acquisition of Phycoerythrin from Porphyridium purpureum
来源期刊	农业生物技术:英文版	学科	农学
关键词	PHYCOERYTHRIN EXTRACTION PURIFICATION IDENTIFICATION
年，卷（期）	2019,（2）	所属期刊栏目
研究方向		页码范围	149-152
页数	4页	分类号	S
字数		语种
DOI