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Translational medical mycology guides clinical and laboratory practice on fungal diseases
Translational medical mycology guides clinical and laboratory practice on fungal diseases
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摘要：
Patients with fungal infection having skin lesions may consult a dermatologist,which is a diagnostic and therapeutic challenge.Dermatologists take samples from the lesion to check the fungal elements under a microscope by KOH preparation and then treat the patient.This model has advanced from bedside to bench and from bench to bedside(B to B to B),which is defined as Translational Medical Mycology.Dermatologists have an advantageous position in finding,isolating and identifying the pathogenic fungi and treating the patient with antifungal drugs.Samples should be cultured in different media with or without chloramphenicol and cycloheximide and incubated at room temperature or 37℃.Non-culture techniques such as polymerase chain reaction based molecular identification,transmission electron microscopy,scanning electron microscopy,biochemistry tests and histopathology are also necessary to confirm the identification of the species,especially when the routine culture is negative.We start treatment upon obtaining evidence of fungal infection,i.e.,positive KOH examination.Antifungal drugs such as itraconazole,fluconazole,terbinafine and amphotericin B can be used alone or in combination based on the fungal species and the location of the lesion.Practice on fungal infection includes screening of the patient,merging all of the laboratory techniques and methods from the microbiologists,pathologists,molecular researchers,identification of the pathogen and determination of the optimum antifungal drug.
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TRANSLATIONAL
MYCOLOGY
BEDSIDE
BENCH
FUNGAL
infections
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世界转化医学杂志
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百世登出版集团有限公司
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其它
ISSN：
2220-6132
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出版地：
北京市朝阳区东四环中路62号楼远洋国际中
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48
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0
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