[Objective] The aim was to study on influence of ultrasonic on broken effect of foodborne polluting strains.[Method] Escherichia coli,Staphylococcus aureus,and Aspergillus niger in different concentrations were tested in different ultrasonic powers and times for the broken effects.[Result] Escherichia coli and Staphylococcus aureus were more sensitive to ultrasonication and the broken rates were as high as 96%.Aspergillus niger,however,was not so sensitive and the broken rate was only about 40%.The optimum parameters,including ultrasonic power,ultrasonic time and OD,of Escherichia coli were 600 W,14 min and 0.997,of Staphylococcus aureus were 600 W,15 min and 1.440,and of Aspergillus niger were 700 W,20 min and 0.893,respectively.[Conclusion] The research provided references for application of ultrasonic technology in food sterilization.