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Standardizing a simpler,more sensitive and accurate tail bleeding assay in mice
Standardizing a simpler,more sensitive and accurate tail bleeding assay in mice
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摘要：
simple,sensitive and accurate bleeding assay.METHODS:Bleeding assay was performed in mice by tail tip amputation,immersing the tail in saline at 37℃,continuously monitoring bleeding patterns and measuring bleeding volume from changes in the body weight.Sensitivity and extent of variation of bleeding time and bleeding volume were compared in mice treated with the P2Y receptor inhibitor prasugrel at various doses or in mice deficient of FcRγ,a signaling protein of the glycoprotein VI receptor.RESULTS:We described details of the bleeding assay with the aim of standardizing this commonly used assay.The bleeding assay detailed here was simple to operate and permitted continuous monitoring of bleeding pattern and detection of re-bleeding.We also reported a simple and accurate way of quantifying bleeding volume from changes in the body weight,which correlated well with chemical assay of hemoglobin levels(r 2=0.990,P<0.0001).We determined by tail bleeding assay the dose-effect relation of the anti-platelet drug prasugrel from 0.015 to 5 mg/kg.Our results showed that the correlation of bleeding time and volume was unsatisfactory and that compared with the bleeding time,bleeding volume was more sensitive in detecting a partial inhibition of platelet’s haemostatic activity(P<0.01).Similarly,in mice with genetic disruption of FcRγas a signaling molecule of P-selectin glycoprotein ligand-1 leading to platelet dysfunction,both increased bleeding volume and repeated bleeding pattern defined the phenotype of the knockout mice better than that of a prolonged bleeding time.CONCLUSION:Determination of bleeding pattern and bleeding volume,in addition to bleeding time,improved the sensitivity and accuracy of this assay,particularly when platelet function is partially inhibited.
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Mouse
or
MICE
TAIL
BLEEDING
ASSAY
PRASUGREL
Platelets
HEMOSTASIS
FcRγ
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研究分支
研究去脉
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世界实验医学杂志
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百世登出版集团有限公司
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不定期
ISSN：
2220-315X
CN：
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出版地：
北京市朝阳区东四环中路62号楼远洋国际中
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90
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0
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0
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